Ann Chambers, PhD

Ann Chambers

Distinguished Oncology Scientist: London Regional Cancer Program, London Health Sciences Centre, London, Ontario

Director: Pamela Greenaway-Kohlmeier Translational Breast Cancer Research Unit

Canada Research Chair in Oncology: Canada Research Chairs

Professor of Oncology: University of Western Ontario, London, Ontario

Cross Appointments: Medical Biophysics and Pathology

Mailing Address

London Regional Cancer Program
Room A4-903b
Cancer Research Laboratory Program
790 Commissioners Rd. E.
London, Ontario
Canada N6A 4L6

Tel: 519.685.8652
Fax: 519.685.8646
Email: ann.chambers@lhsc.on.ca

Research Area

Cell and molecular biology of metastasis

Summary of Current Work

Metastasis, the spread of cancer cells from a primary tumor to new sites, is a major factor in preventing successful treatment of cancer. Metastasis can occur after years of dormancy following treatment of a primary cancer. We are using experimental and clinical approaches to study metastasis and tumor dormancy. We have used in vivo videomicroscopy to clarify the steps and molecular mechanisms in metastasis. We have discovered that large numbers of dormant single cells may remain in secondary organs, with the potential to resume growth at later times to form metastases. We also are using in vitro models to study the molecular regulation of tumor dormancy. Our research suggests that new anti-metastatic therapies should be directed against the site-specific growth of cancer cells after they have arrived in the new organ. We are collaborating with Dr. Paula Foster to use novel cellular magnetic resonance imaging approaches to study metastasis and tumor dormancy.

We also are studying how an oncogene-induced, integrin-binding protein called osteopontin (OPN) contributes to the growth and progression of many kinds of tumors, in collaboration with Dr. Alan Tuck. We have shown that OPN can function to promote malignancy of cells in culture, and we are studying how OPN affects tumor growth and progression. We have developed an ELISA that can measure OPN plasma levels in patients. In clinical studies, we have shown that women with metastatic breast cancer, as well as men with castrate resistant prostate cancer, have blood OPN levels that are higher than normal levels, and that OPN tissue levels are higher in many types of tumors than in adjacent normal tissue. Elevated OPN levels are associated with poorer survival. These experimental and clinical studies will clarify the role of OPN functionally in cancer, and its potential role as a prognostic marker in breast, prostate and other cancers.

Also in collaboration with Dr. Tuck, we are studying molecular determinants of early breast cancer progression. Using in vitro and in vivo models, we have identified a series of genes whose expression changes as breast cells progress from atypical ductal hyperplasia, to ductal carcinoma in situ, to invasive mammary carcinoma.

The overall aim of our research is to learn how cancer cells spread, in order that new approaches to prevent, delay or treat development of metastatic disease can be developed.

PubMed Publications

See my publications on PubMed

View Publication (PDF)

Goss PE, Chambers AF. Does tumour dormancy offer a therapeutic target? Nature Reviews Cancer 10: 871-877, 2010.

View Publication (PDF)

Chambers AF, AC Groom and IC MacDonald. Dissemination and growth of cancer cells in metastatic sites. Nature Reviews Cancer 2: 563-572, 2002.

Key Collaborators

Alan B. Tuck, MD, PhD, FRCPC (Anatomic Pathology)
Departments of Pathology and Oncology, University of Western Ontario
Staff Pathologist: Department of Pathology, London Health Sciences Centre

Dr. Tuck is a pathologist, specializing in breast pathology, whose clinical work is based at London Health Sciences Center and St. Joseph's Health Centre, also affiliated with the London Regional Cancer Program. He is a consultant in breast pathology for SW Ontario. Research interests include the cell and molecular biology of breast cancer, osteopontin, tumor metastasis, and early development of models for studying breast cancer progression. This research is translational in nature, with the goal of developing new tools/targets for the management of patients at different stages of breast cancer.

Paula Foster http://www.robarts.ca/paula-foster

Robarts Research Institute, University of Western Ontario

Staff and Research Trainees

Research Associate:

Dr. Pieter Anborgh
Project: Experimental and clinical studies on the role of osteopontin in cancer
Email Dr. Pieter Anborgh

Postdoctoral Fellow:

Hon Sing Leong, PhD

Co-supervisor: Dr. John Lewis
Project: Novel imaging approaches and molecular regulation of metastasis
Email Hon Sing Leong

Graduate Students:

Connor MacMillan

MSc student, Pathology Department

Joint supervisors: Drs. Alan Tuck and Ann Chambers
Project: Molecular regulators of early breast cancer progression
Email Connor MacMillan

Allen Clifford

MSc student, Pathology Department
Joint supervisors: Drs. Alan Tuck and Ann Chambers
Project: The role of the transcription factor TBX3 in early breast cancer progression
Email Allen Clifford

Caroline Trieman

MSc student, Pathology Department
Joint supervisors: Drs. Alan Tuck and Ann Chambers
Project: The role of S100A2 in early breast cancer progression
Email Caroline Trieman

Phil Medeiros

PhD student, Medical Biophysics Department

Supervisor: Dr. Dwayne Jackson
Co-supervisor: Dr. Ann Chambers
Project: The role of the sympathetic nervous system on breast cancer progression
Email Phil Medeiros

Matthew Lowerison

MSc student, Medical Biophysics Department

Supervisor: Dr. Jim Lacefield

Co-supervisor: Dr. Ann Chambers

Project: Power Doppler ultrasound and angiogenesis

Email: Matthew Lowerison

Muriel Brackstone, MD

PhD student, Pathology (part-time)

Project: Concurrent neoadjuvant chemo/radiation for locally advanced breast cancer – understanding and predicting treatment resistance.

Email: Dr. Muriel Brackstone

Research Technicians:

David Dales, BSc (Honours Genetics), Senior Research Technician
Specialty: Molecular and cellular biology and overall laboratory management
Email David Dales

Nicole Hague, BSc, RVT

Specialty: Veterinary technician

Email Nicole Hague

Carl Postenka, BSc, M.L.T., Histology Research Technician
Specialty: Animal handling and all aspects of histology
Email Carl Postenka

Joseph Andrews, Bsc, MSc, Research Technician

Specialty: Microarray technology, molecular biology; methylation studies (with Dr. David Rodenhiser)

Email Joseph Andrews